Cannabinoid sample assessment: Concentrated or Diluted sample

SAMPLE ASSESSMENT

Whether your sample has a low or high rate of cannabinoids, you can calculate a multiplication factor in order to be able to test a low THC rate from hemp or a high rate from concentrate. Just apply this simple rule based on the weight of your sample:

Multiplication factor = (100 mg x 2µl) / samples weight (mg) X extraction fluid (µl)

Concentrated samples (high cannabinoid concentration)

In case of high concentration samples where you expect between 40% and 100% CBD for example you will take 40mg of samples with 1µl extraction fluid with the suitable capillary tube then:
Multiplication factor = (100 mg x 2ul) / 40 (mg) X 1 (µl) = 5
Then after spot calibration of the plate, if your chart shows 13% CBD multiply by 5, you will actually have 65% CBD in your sample.

Diluted samples (low cannabinoid concentration)

In a case of low concentrate samples where you expect between 0.1% and 5% THC for example, you will take 200mg of samples with 8µl extraction fluid with the suitable capillary tube, then :
Multiplication factor = (100 mg x 2ul) / 200 (mg) X 8 (µl) = 0.125
Then after spot calibration of the plate, if your chart shows 5% THC multiply by 0.125, you will actually have 0.6% THC in your sample.

See more examples in the tables below:

Potency testing for cannabinoid samples from 1 to 40% (Flowers, Hash, other, …) Potency testing (4 sample product / plate)

	Lane 1	Lane 2	Lane 3	Lane 4
Sample Size	100mg	100mg	100mg	100mg
Sample Solution	1*µl	1*µl	1*µl	1*µl
Print Lane	Heated	Heated	Heated	Heated
Multiplication Factor	2	2	2	2
Analyze	From 1 to 40% CBD, CBN, THC, THCV, CBG, CBC

Chemotype testing for main and secondary cannabinoid (CBN, THCV, CBG, CBC) for sample below 40% THC/CBD testing + Acid form (1 sample product/plate)

	Lane 1	Lane 2	Lane 3
Sample Size	100mg	100mg	100mg
Sample Solution	2*µl	1*µl	2*µl
Print Lane	Heated	Heated	Raw
Multiplication Factor	1	2	1
Analyze	From 1 to 20% CBD, CBN, THC, THCV, CBG, CBC	From 20 to 100% CBD, CBN, THC, THCV, CBG, CBC	Lane 1 – Lane 3 = Acid Form (THCA,CBDA…)

How to measure the cannabinoid acid form?

For exemple: if you obtain 16% THC (LANE 1) and 5% THC (LANE 3), then 16% – 5% = 11% of THCA in your sample.

Chemotype testing for main (THC, CBD) and secondary cannabinoid (CBN, THCV, CBG, CBC) for samples above 40% (Concentrated oil, Ice O lator…) Potency testing + Acid form (1 sample product/plate)

	Lane 1	Lane 2	Lane 3
Sample Size	40mg	40mg	40mg
Sample Solution	2*µl	1*µl	2*µl
Print Lane	Heated	Heated	Raw
Multiplication Factor	2,5	5	2,5
Analyze	From 1 to 20% CBD, CBN, THC, THCV, CBG, CBC	From 20 to 100% CBD, CBN, THC, THCV, CBG, CBC	Lane 1 – Lane 3 = Acid Form (THCA,CBDA…)

How to measure the cannabinoid acid form?
For exemple: if you obtain 66% THC (LANE 1) and 15% THC (LANE 3), then 66% – 15% = 51% of THCA in your sample.

Concentrated sample: For accurate results make sure to use a high precision scale to weigh a minimum of 1mg (0.001g).
Without you cannot guarantee the exact percentage of the results.

Chemotype testing for cannabinoid samples below 1% (Hemp) Potency testing + Acid form (1 sample product/plate)

	Lane 1	Lane 2	Lane 3
Sample Size	200mg	200mg	200mg
Sample Solution	2*µl	8*µl	2*µl
Print Lane	Heated	Heated	Raw
Multiplication Factor	0.5	0.125	0.5
Analyze	From 1 to 20% CBD, CBN, THC, THCV, CBG, CBC	From 0 to 1% CBD, CBN, THC, THCV, CBG, CBC	Lane 1 – Lane 3 = Acid Form (THCA,CBDA…)

How to measure the cannabinoid acid form?
For example: if you obtain 1% THC (LANE 1) and 0,5% THC (LANE 3), then 1% – 0,5% = 0,5% of THCA in your sample.

How to conduct cannabinoid testing without sending samples to a laboratory?

You can conduct cannabinoid tests by yourself (without sending samples to a lab) with Alpha-cat Cannabinoid Testing Kits.

Our testing kits are in full compliance with the guidelines of the World Health Organization concerning the safety and quality of medicinal plant materials (WHO/PHARM/92.559) and can be used to detect the presence of 6 cannabinoids (CBD, CBN, THC, THCV, CBG, CBC).

The Alpha-cat Mini Cannabinoid Testing Kit includes 8 test kits and our Regular Cannabinoid Testing Kit has 40 test kits.